Hyperspectral Optical Microscopy

CytoViva’s enhanced darkfield microscope optics improve signal-to-noise up to ten times (10x) over standard darkfield optics. This enables nanomaterials as small as 10nm-20nm to be imaged right from your laboratory benchtop.

CytoViva’s patented (US patents No. 7,542,203, 7,564,623) enhanced darkfield illumination system, which replaces the standard microscope condenser, works by coupling the source illumination directly to the condenser optics.

Producer: CytoViva, USA

A modular configuration which allows the users to:

With the Enhanced Illumination Module: CytoViva’s enhanced darkfield microscopy enables scientists to optically observe a wide range of nanoscale materials quickly and easily in solution, live cells, tissue and materials based matrices. In addition, non-fluorescent live cells and pathogens can be easily observed at a level of detail not possible with traditional optical imaging techniques such as phase contrast or differential interference contrast. Download specifications here (PDF)
The Dual Mode Fluorescence (DMF) Module allows for the observation of both fluorescent and non-fluorescent sample portions simultaneously and in real-time. Samples are viewed directly through the microscope eyepiece and captured using a standard microscope camera without the need for complicated software or electronic manipulation.

The Hyperspectral Imagining (HSI)
Works even in Visible Near Infrared, 400 nm – 1.000 nm (VNIR) or in Short Wave Infrared, 900 nm – 1,700 nm (SWIR) – according to the customer’s applications.
Was specifically developed for spectral characterization and spectral mapping of nanoscale samples imaged with the patented CytoViva darkfield-based microscope.

Hyperspectral image means that each pixel of a hyperspectral image provides the complete reflectance spectral response of that pixel’s spatial area within the VNIR or SWIR spectral range.

At 100x magnification, a hyperspectral microscope image may contain as many as 700,000 pixels, each as small as 128nm each.
2nm spectral resolution
Hyperspectral microscope images can be captured of both biological and materials-based nanoscale samples. These nanoscale materials may be integrated in a wide range of biological or materials based environments.
Fast imagining time: up to 3-5 minutes
>> Download specifications here (PDF)
CytoViva 3D Enhanced Darkfield Imaging System
Provides a method for locating non-labeled nanostructures (particles, tubes, etc.) in a variety of translucent matrices (cells, tissue, organisms).
This technique leverages the high signal-to-noise optical performance in combination with patent-pending deconvolution and particle location routines to provide users with a three dimensional optical model of their sample.
This technique does not require the use of fluorescent labels on the nanoparticles to obtain an image of the particles, thus removing the potentially negative influence of these labels on the sample.
Designed to facilitate research in a variety of applications in nanobiology (targeted drug delivery and pathogen detection), nanotoxicology (nanoparticles and carbon nanotubes in tissue), and others where it is useful to understand the location of nanostructures in a three dimensional space.

Live Cell Environmental Chamber
Live cell, microfluidics and simulated in-vivo studies can be accomplished when using the CytoViva imaging system coupled with the SynVivo environmental chamber.
In combination with the CytoViva imaging system, the SynVivo system is used to track nanoparticles as they interact with live cells and tissue in drug delivery and related assays.

Integrated Raman with Hyperspectral Microscopy
HORIBA Scientific and CytoViva have integrated confocal Raman imaging, hyperspectral imaging and enhanced darkfield imaging onto the same microscope platform.
Combining three different rapid imaging methods allows an easy observation, characterization and identification on the nanoscale.
Download specifications here (PDF)

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