Discover the physiological phenomena of living cells at nanoscale. Delicate membrane morphology imaging at cellular and sub-cellular level now possible with the SICM head (Scanning Ion Conductance Microscopy)
Park NX12 is a powerful 3-in-1 Bio-Research tool that uniquely combines SICM with AFM and an inverted optical microscope (IOM) on the same platform.
- Designed for non-invasive in-liquid imaging, Park NX12 is the ideal tool for studying biological materials under physiological conditions.
- It combines the bio-mechanical property measurement capability of the AFM and nano imaging of the SICM in liquid, and the optical viewing of the IOM
- The modular design of the Park NX12 allows researchers to easily switch between its SICM and AFM capabilities.
About SICM Technology
- Park SICM uses nanopipettes instead of the classical AFM tip
- In Scanning Ion Conductance Microscopy developed by Park Systems (Park SICM), a glass nanopipette filled with an electrolyte acts as an ion sensor that provides feedback on its location relative to a sample completely immersed in liquid. The pipette tip maintains its distance from the sample by keeping the ionic current constant.
In comparison, AFM typically relies on interaction of forces between its probe tip and the sample.
- Park SICM can acquire biological images at nanoscale in physiological conditions, attaining high resolution of less than 200 nm. The biological images obtained from SICM are free from morphological deformation, which can occur from scanning electron microscopy (SEM) or even AFM systems.
- No Force, Non-Contact Imaging in Liquid
The SICM operates in liquid without making physical contact with the sample. Electrodes on either side of the sample and pipette produce ionic current that flows through the surrounding solution. A sensor measures the current flow, which decreases as the distance between the pipette and sample becomes smaller, and monitors the distance between the pipette and the sample to obtain the topology.
- Park SICM Can Image All Cell Types – Park SICM can image even the softest cells such as the neuron cells, live – something that’s impossible with any other microscopy techniques.
About AFM Technology
- Enables Accurate Force-distance Spectroscopy
Park AFM’s industry leading low noise Z detector allows the researcher to control Z scanner movement to apply an exact amount of force very accurately to a sample surface during the FD spectroscopy.
Force-distance (FD) spectroscopy using an AFM is a beneficial tool to characterize bio-mechanical properties (nano-newton scale) of various biological materials.
- Advanced Bio-mechanical Property Measurement by Calculating Elastic Modulus (Young’s Modulus)
The Herzian and Oliver Pharr models are calculated automatically from the Park AFM’s accurate FD spectroscopy data to determine the elastic modulus (Young’s modulus). Both of these calculation methods are included in Park XEI, the data analysis software in Park NX12.
Outstanding Investigation Tool for Biological Research by Combining Physiological Morphology with Bio-Mechanical Property Measurements
Park NX12 combines Park SICM’s ability to interpret morphology under true physiological conditions and Park AFM’s capacity to acquire bio-mechanical property data (elastic modulus) accurately.
Live Cell Study with Live Cell Chamber (SICM & AFM)
- Creates an ideal environment for cells, improving their life expectancy during long measurement durations through controlled temperature, pH, and humidity at optimal conditions.
Ion Channel Recording of Targeted Patch Clamping
- Targeted Patch Clamping is the SICM-based version of this technique that enables the detection of ion channel activities of specific subcellular structures.
SICM+Patch Clamping=Targeted Patch Clamping
Integrating Fluorescence Microcopy with Park SICM
- Combining fluorescence microscopy (FM) techniques with Park SICM can create new benefits and provide comprehensive information for cell biology studies that cannot be obtained when using only one of those techniques. While monitoring external cellular surface morphology with SICM, the internal cellular behavior can be observed by FM.
SmartScanTM – Data Acquisition
- SmartScanTM is a data acquisition software that provides all user controls of Park AFM measurements. The friendly user-oriented interface of SmartScanTM provides easy operation of the AFM.
- Simultaneous data acquisition of up to 16 images
XEI – Image Processing and Analysis– With its most advanced and versatile imaging features, AFM users can obtain essential and critical information from their experiment.
- Image Overlay: SICM Topography + Fluorescence Microscopic Image